Journal: STAR Protocols

Article Title: Protocol to measure temporal consumption and feeding choices of Drosophila adults using capillaries

doi: 10.1016/j.xpro.2025.103931

Figure Lengend Snippet: Fill the capillaries and align them on a microscope glass slide The capillaries are filled by capillarity when plunged into the solution within an Eppendorf. Take each capillary out of the Eppendorf and dry the outer surface by rolling it on a sheet of paper. The capillaries can be handled with forceps. Align the capillaries parallel to each other on a 3D-printed template to transfer them onto a microscope slide. (A) Material needed: (1) a multiCAFE template, (2) one microscope slide, (3) double sided sticky tape, (4) fine forceps to handle capillaries, (5) capillaries, (6) test solution 1 and (7) test solution 2. (B) Insert capillaries in the Eppendorf tubes. Catch one capillary at a time and roll it over a sheet of paper to dry it externally. Then position it in one of the grooves of the template. Do it sequentially so that all capillaries filled with solution 1 are on the left of each cage and all capillaries of solution 2 are on the right. (C) Place double-sided sticky tape over the length of the microscope slide and cut the sides. (D) Turn the slide over and position it over the capillaries that have been aligned previously. Press gently the glass slide to make the capillaries stick to the tape and then remove the slide. If some capillaries are slightly mis-oriented realign them with forceps or repeat the operation.

Article Snippet: For this step, you will need one cage template, one microscope slide, double sided sticky tape, forceps, 5 μL capillary tubes (n=20), and two 1.5 Eppendorf tubes with your solutions ( A).

Techniques: Microscopy